Abstract of research

SEEKING BETTER SEROLOGICAL MEANS FOR ACCURATE DIAGNOSIS OF RUMINANT BRUCELLOSIS

Abdel Hamid, N.H.a, Ebeid, M.H.b, Arnaout, F.K.b, Elgarhy, M.M.c, Elbauomy, E.M.a and Sayour, A.E.a

Abstract

In this investigation, animals were classified by a panel of immunoassays into Brucella true positives and true negatives. Agreement with complement fixation test (CFT) was used to assess three versions of enzyme-linked immunosorbent assay. Competitive ELISA with lipopolysaccharide antigen (cELISA-LPS) achieved the highest agreement (96.7%) then indirect ELISA using periplasmic protein antigen, iELISA-PPP, (95.8%) and indirect ELISA with LPS antigen, iELISA-LPS, (94.7%). The highest sensitivity was shown by iELISA-LPS (92.4%) and buffered acidified plate antigen (BAPA) test (92.3%), then by cELISA-LPS (91.2%) and iELISA-PPP (90.6%). Brucellosis card test (BCT) (86%) and CFT (85.9%) had almost identical sensitivity. Microtiter plate agglutination test, PAT, (78.7%) was the least sensitive. The highest specificity was revealed by cELISA-LPS (96.23%) then CFT (92.4%), iELISA-PPP (91.6%) and iELISA-LPS (89.5%). BCT and BAPA revealed similar figures of 88.5% and 87.1% respectively. PAT was the least specific (77.6%). The efficiency estimates of immunoassays were maximal in cattle (96.9%), and slightly lower in buffaloes (96.5%) and sheep (96.2%) with some decrease in goats (95 %).