EVALUATION OF THE EFFICACY OF DIFFERENT FORMULAE OF
CONCENTRATED INACTIVATED RABIES VACCINE
Amany A. Abassa, El-Bagory, G.F.b and Khodeir, M.H.a
Abstract
There is increased cases of rabies worldwide and be afraid in everywhere. Such vaccine aimed to
induce high level of more prolonged immunity in a safe manner. So the present work evaluate all
prepared vaccine formulae which from different adjuvant and different doses and also by concentrated
virus by PEG6000, were found free from foreign contaminants and safe induce no abnormal general
or local clinical sings .The induced rabies antibodies were followed up in the sera of each puppies up
to one year using serum neutralization test (SNT), indirect enzyme linked immune sorbent assay
(ELISA). Both of SNT and ELISA indicated that the highest antibody level was induced by the
vaccine adjuvant montanide ISA206 and inactivated by beta propiolactone and concentrated by poly
ethylene glycol 6000.
Key words
Montanide ISA206, PEG, SNT.
